Monday, January 3, 2011
Inserting Sample on NMR Magnet
1. NMR tube containing sample should be held in a plastic spinner (use the blue color spinner in 500 and 600 MHz spectrometers; use the white color spinner in 700 MHz spectrometer). Hold the sample by the top, place sample tube in the spinner and the spinner in the sample depth gauge. Push or pull the sample tube so that the depth of the sample above and below the center line of the sample depth gauge is equal. However, never exceed the lower limit (position of the adjustable white platform which should be at the line marked 5 mm 15mm) as this can damage the probe as well as the sample, as the sample will rest on the tip of the tube, not the spinner. This is important for all probes, but especially the cryoprobes installed on the 600 and 700 MHz spectrometers.
2. Remove the black cap from the top of the magnet bore. Next, press the LIFT button in upper left portion of the BSMS keyboard. Wait for the airflow (hissing sound that can be heard). Then remove the depth gauge before inserting the sample and spinner into the magnet. Pressing the LIFT button will toggle the air flow off and will drop the sample tube gently to the magnet bore where it will be positioned at the top of the probe.