(1) Add the sample to be injected to the syringe.A 25µL glass Hamilton syringe is used to inject the GC samples. Only 2-4 µL of sample is injected onto the column, which means that you fill only a small part of the barrel with sample. Examine the syringe carefully before you fill it. The divisions are marked "5 - 10 - 15 - 20 - 25".
(2) Inject the sample into the injector port.You are need to do two things sequentially and quickly, so make sure you know where the injection port is and where the start button on the recorder is.
Push the needle of the syringe through the injection port and immediately press the plunger to inject the sample, then immediately press the start button on the recorder.
You will feel a bit of resistance from the rubber septum in the injection port; this is to be expected and you should be prepared to apply some pressure to the syringe as you force the needle into the instrument all the way to the base of the needle.
(3) Sit back and wait.
(4) End the GC runWhen you have seen all of the peaks which you suspect are in the mixture, or when the recorder has shown a flat baseline for a few minutes or so, press stop on the recorder.
When you press stop, the recorder will print out the peaks, the retention times, and the areas under the peaks. When it is done printing, you can press “enter” a couple times to advance the paper.
Carefully tear the paper off the recorder. The paper is not perforated, so do not try to pull up and expect it to pop out of the recorder. Instead, pull it down to start a tear from one edge, and then continue the tear until the paper is cut and free