| Disease | Medicine |
| Detector off | Check Detector |
| Broken connections to recorder | Check connections |
| No flow | Check flow |
| No sample/Wrong sample | Check sample. Be sure it is not deteriorated. Check for bubbles in the vials |
| Wrong settings on recorder or detector | Check attenuation. Check gain |
| Disease | Medicine |
| Pump off | Start pump |
| Flow interrupted | Check reservoirs. Check position of the inlet tubing Check loop for obstruction or air Check degasing of mobile phase Check compatibility of the mobile phase components |
| Leak | Check fittings Check pump for leaks and precipitates Check pump seals |
| Air trapped in the system | Disconnect column and prime pump Flush system with 100% methanol or isopropanol Contact servicing if necessary |
Reference:
Prof. Dr. H.J. Chaves das Neves
Universidade Nova de Lisboa
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