Monday, December 20, 2010

No Pressure or Pressure Lower than anticipated

No Pressure or Pressure Lower than anticipated

What you Expect.                                 What you got

Check for loose fittings
Check pump for leaks, salts and noise
Check seals
Mobile phase interrupted
Check reservoirs. Check position of the inlet tubing
Check loop for obstruction or air
Check degasing of mobile phase
Check compatibility of the mobile phase components
Air trapped in pump
Check flow
disconnect column and prime pump
Flush system with 100% methanol or iso propanol
Contact servicing if necessary
Worn pump seals
Replace seals
Check pistons and replace if necessary

Higher Pressure than anticipated

  What you Expect.                                 What you got

Pump, injector, tubing
Disconnect column, run pump at 25 mL/min: Is the pressure minimal? Go to next step
If pressure still high check systematically from detector to pump for obstruction
If guard column is obstructed, replace it
If the analytical column is obstructed reverse it and flush disconnected from the detector. Use adequate restoration procedures
Change inlet frit
Replace de column

Prof. Dr. H.J. Chaves das Neves
Universidade Nova de Lisboa 

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