Monday, December 20, 2010

Retention times are not constant

Retention times are not constant

What you Expect                                 What you Got                                         

Check for loose fittings
Check pump for leaks, salts and noise
Check seals
Change in mobile phase composition
Check. Make new
Air trapped in pump
Check flow
Prime pump. Check and change seals. Be sure the mobile phase is degasse
Temperature fluctuations
Stabilise column temperature. Use column oven
Dilute sample
Sample dissolved in a solvent that is incompatible with the mobile phase
Dissolve sample in the mobile phase whenever possible. Adjust

Prof. Dr. H.J. Chaves das Neves
Universidade Nova de Lisboa 

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