What you Expect What you Got
| Disease | Medicine |
| Leak | Check for loose fittings Check pump for leaks, salts and noise Check seals |
| Change in mobile phase composition | Check. Make new |
| Air trapped in pump | Check flow Prime pump. Check and change seals. Be sure the mobile phase is degasse |
| Temperature fluctuations | Stabilise column temperature. Use column oven |
| Dilute sample | |
| Sample dissolved in a solvent that is incompatible with the mobile phase | Dissolve sample in the mobile phase whenever possible. Adjust |
Reference:
Prof. Dr. H.J. Chaves das Neves
Universidade Nova de Lisboa
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